LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Aqueous solutions of NMA, Na2HPO4, and NaH2PO4 as models for interaction studies in phosphate–protein systems

Photo from archive.org

Abstract Phosphate buffers are essential for many areas of studies. However, their influence on buffered systems is often ignored. The phosphate salts can interact with biologically important macromolecules (e.g. proteins)… Click to show full abstract

Abstract Phosphate buffers are essential for many areas of studies. However, their influence on buffered systems is often ignored. The phosphate salts can interact with biologically important macromolecules (e.g. proteins) and stabilize or destabilize them. With our research, we want to answer question what kind of interactions, if any, occur between phosphate ions and a protein backbone model — N-methylacetamide (NMA). ATR-FTIR spectroscopy in the amide I range and in the regions characteristic for P O vibrations provides information on direct and indirect (water-mediated) interactions. The analysis is supported by chemometric, DFT, and QTAIM calculations. Our results indicate that direct NMA–phosphate ion interactions are quite rare and indirect. Water molecules seem to play an important role in such systems. The model studies indicate that no preferential interactions between NMA and phosphate ions in solutions are formed, and may imply that such interactions are also unfavorable in protein-based systems.

Keywords: aqueous solutions; solutions nma; nah2po4 models; na2hpo4 nah2po4; models interaction; nma na2hpo4

Journal Title: Journal of Molecular Liquids
Year Published: 2018

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.