Photo by nci from unsplash
Forward genetic screens have been instrumental in deciphering the molecular mechanisms that control agriculturally important traits. Traditionally, crop mutant collections are generated by chemical mutagenesis such as ethyl methanesulfonate (EMS)… Click to show full abstract
Forward genetic screens have been instrumental in deciphering the molecular mechanisms that control agriculturally important traits. Traditionally, crop mutant collections are generated by chemical mutagenesis such as ethyl methanesulfonate (EMS) treatment, physical irradiation, and insertional agents including T-DNAs and transposons (Wei et al., 2013; Li et al., 2016). Because EMS and irradiation-generated mutations are mosaic at the first generation (M1), forward genetic screens for any desirable phenotypes have to be conducted at the M2 generation.
Journal Title: Molecular plant
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!