LAUSR

Abstract A novel collagen matrix was developed to accelerate wound healing for hard to heal or delayed wound healing clinical conditions. It is produced from porcine skin, chemically comparable and biocompatible to the human skin. Supercritical carbon dioxide (SCCO2) was employed to decellularize porcine skin to produce collagen matrix (CM). To confirm the complete decellularization of the CM, hematoxylin and eosin staining, and DNA contents were analyzed. In addition, we analyzed the chemical content and structural property. In vitro, in vivo toxicity and biocompatibility was evaluated for CM. In addition, wound healing was evaluated in a porcine excision full-thickness skin wound model mimicking human wound healing. SCCO2 produced CM exhibited complete decellularization, the chemical content was found to be type I collagen and characteristic features were similar to that of human. CM was found to be non-toxic in all the toxicological tests. In addition, CM exhibited excellent biocompatibility in both in vitro and in vivo studies. In porcine excision full-thickness skin wound healing model, CM alone and cocultured with fibroblast and keratinocytes exhibited decreased inflammation, complete epithelization and enhanced wound healing. To conclude, CM produced by SCCO2 technology revealed chemically similar to human skin type I collagen, non-toxic, good biocompatibility and accelerated wound healing in porcine excision full-thickness skin wound model. Statement of significance As far as our knowledge, we are the first to report the SCCO2 extraction technology to decellularize the porcine skin to produce and characterize collagen matrix. We evaluated in vitro and in vivo biocompatibility of the collagen matrix. Furthermore, we investigated the regenerative efficacy of collagen matrix on excision full-thickness skin wound healing model in the pig. We proved the complete decellularization of porcine skin by SCCO2 extraction technology to produce collagen matrix. In addition, it demonstrated to be excellently biocompatible in both in vitro and in vivo tests, without adverse effects. The present study demonstrated that the collagen matrix developed by SCCO2 extraction technology could be a perfect and beneficial wound healing and regenerative matrix in tissue engineering.