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s / Placenta 83 (2019) e1ee118 e84 spectroscopy provides a plethora of useful information regarding biochemical changes in atomic level. They already have several applications in cancer research and diagnosis, but could be important tools to help all pregnancy and placenta fields of study. Therefore, we aimed to analyze possible biomechanical and biochemical alterations in trophoblast cells incubated with Group B Streptococcus (GBS). Methods: HTR-8/SVneo cells were incubated with inactivated serotype Ia GBS. F-actin cytoskeleton was stained by phalloidin and AFM was used to access cellular morphology, height, rugosity and elasticity, the latter by Young’s Modulus calculation. Raman spectroscopy was employed and principal component analysis (PCA) and linear discriminant analysis (LDA) were applied to search the best cell classification and most significant biochemical differences among raman spectra. Results: GBS at 108 CFU altered F-actin cytoskeleton, as cells lost stress fibers and migratory phenotype, becoming roundish with F-actin aggregates. Topographic images depicted cells with a rougher surface after GBS incubation. Membrane elasticity was increased with GBS incubation, while uvaol increased cellular stiffness. PCA and LDA analysis showed GBS increased cytochrome C resonant raman signals (700-750 cm-1, 1311-1585 cm-1 peaks), as well as peaks of phosphatidylserine and phosphodiester, which strongly correlates to apoptosis and necrosis signals, while control cells had higher 608-614 cm-1 peaks of cholesterol, 847-856 cm-1 peaks of carbohydrates and 1000 cm-1 NADH peak, which are related to aerobic and lipid metabolism. Conclusion: AFM and Raman findings strongly suggest GBS increase cell death, decrease cell migration and metabolism. As such, they are powerful tools that can be extremely accurate in finding cellular atomic events and could help on diagnosis and even undercover hidden changes in placental pathologies. P2.55. STRUCTURE-FUNCTION RELATIONSHIPS IN THE FETO-PLACENTAL CIRCULATION FROM IN SILICO INTERPRETATION OF MICRO-CT VASCULAR STRUCTURES Monika Byrne , Rosalind Aughwane , Joanna James , Ciaran Hutchinson , Owen Arthurs , Neil Sebire , Sebastien Ourselin , Anna David , Andrew Melbourne , Alys Clark . Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand; 2 EGA Institute for Women's Health, University College London, London, United Kingdom; Great Ormond Street Hospital, London, United Kingdom; Kings College London, London, United Kingdom; University College London, London,