LAUSR

Abstract Mania is characterized by elevated drive and mood but animal models of mania have often neglected elevated mood. Ultrasonic vocalizations (USV) of 50‐kHz emitted by rats are thought to index the subject's positive affective state. Fifty‐kHz USV emission is increased by amphetamine, an effect blocked by lithium administration. Sleep deprivation (SD) is an environmental model of mania and the present study evaluated SD effects on behavioral activity and USV emission, together with the impact of lithium treatment. Adult rats were submitted to 24h or 72h SD, and locomotor activity and USV emission were assessed. To test their sensitivity to a standard antimanic drug, these behavioral parameters were also evaluated after acute administration of lithium carbonate (25, 50 or 100 mg/kg, i.p.). Striatal monoamine content was measured post‐mortem. SD (24h and 72h) led to increased locomotor activity, rearing behavior and 50‐kHz USV emission, together with a change in the call profile characterized by an increase in the percentage of frequency‐modulated 50‐kHz USV, which may indicate the mania‐like consequences of SD. Importantly, all SD effects were reverted by lithium administration. SD also led to a decrease in dopamine content in the ventral striatum, while increasing dopamine turnover. In conclusion, SD increased 50‐kHz USV emission, an effect prevented by acute lithium administration. This suggests 50‐kHz USV as a new marker for mania‐like elevated mood, which shows construct validity (associated with increased dopaminergic tone), face validity (reflecting increased positive affect) and predictive validity (high sensitivity to lithium treatment). Highlights50‐kHz Ultrasonic Vocalizations (USV) are proposed as an index of positive affect.Sleep deprivation increased locomotor activity, rearing and 50‐kHz USV.These effects were blocked by lithium pretreatment.50‐kHz USV can be a marker of elevated mood in animal models of mania.