LAUSR

Abstract Objective To ascertain various phytochemical ingredients in Ximenia americana leaves extracts by GC-MS & evaluating their antidiabetic property by using in vitro assays. Methods The serial extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were subjected to GC-MS analysis and also the antidiabetic activity was assessed by employing standard in vitro techniques. Results GC-MS analysis confirmed the presence of different phytochemicals in each leaf extracts of X. americana . The major phytoconstituents were found to be Oleic acid, n-Hexadecanoic acid, Non-decanoic acid and Octadecatrienoic acid in chloroform extract; and 3-Undecene, Tridecene, Trifluoroaceteoxy tetradecene, and Trichloroacetic acid-3-tridecyl-ester in ethyl acetate extract; where in 1-Tetracosanol, Behenyl alcohol, 1-Hexacosanol, Octadecanal, 4-Piperidine propanoic acid and α –D-mannofuranoside in methanol extract; 8,11,14-Eicosatrienoic acid, 7-Tetradecanal and 1-Ocytyn-3-Ol-4-Ethy in ethanol extract; aqueous extract showed the presence of 9,12-Octadecandionoic acid. In vitro antidiabetic studies show that aqueous extract exhibited significant activity when compared to other solvent extracts. Conclusion The investigation confirms that aqueous extract exhibited highest antidiabetic activity among all extracts; Additional studies on needed for purification, characterization and structural elucidation of bioactive compounds from aqueous extract & also confirm its antidiabetic property by in vivo studies. This study provides scientific evidence that leaves of Ximenia americana have anti-diabetic efficacy. Thus, considering its relative hypoglycemic potency, they may serve as useful therapeutic agents for treating diabetes.