LAUSR

In this study, we have for the first time preformed the facile substrate-enhanced electroless deposition (SEED) of metal nanoparticles onto monolithic [email protected] foams for construction of disposable three-dimensional (3D) electrochemical immunosensors. Specifically, we firstly used the SEED method to deposit gold nanoparticles (AuNPs) onto the [email protected] foam for immobilization of antibody (Ab1). This is followed by a second step SEED deposition to produce silver nanoparticles (AgNPs) for electrochemical stripping detection. Using α-fetoprotein antigen (AFP) as a module analyte, the newly-developed sensor showed a wide linear response, ranging from 5.0 pg/mL to 5.0 ng/mL and a low detection limit down to 2.3 pg/mL. The newly-developed 3D-immunosensor is sensitive, reliable, and easy to be fabricated, showing great potential for clinic applications.