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Identification of S genotypes in loquat (Eriobotrya japonica Lindl.) based on allele specific PCR

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Abstract Loquat (Eriobotrya japonica Lindl) is an evergreen fruit tree and belongs to Maloideae in the Rosaceae, which carries the RNase-dependent gametophytic self-incompatibility system (GSI). Use of appropriate pollinator trees… Click to show full abstract

Abstract Loquat (Eriobotrya japonica Lindl) is an evergreen fruit tree and belongs to Maloideae in the Rosaceae, which carries the RNase-dependent gametophytic self-incompatibility system (GSI). Use of appropriate pollinator trees is essential for increasing fruit production. S-allele specific amplification system is an efficient and rapid method to identify S-genotypes. In this research, according to the sequences of S-RNase alleles, 9 specific primer pairs for amplifying S-RNase alleles (S2, S7, S6, S8, S9, S10, S11,S12 and S13) were designed, which located in the introns or in the areas with high polymorphism. The specificity of the 9 allele specific primer pairs was evaluated by eight loquat cultivars with known S-genotypes. After being verified their effectiveness, they were used to S-genotype 39 loquat cultivars from Longan and loquat nurseries in Fuzhou national fruit germplasm. The results showed that the 39 loquat cultivars were S-genotyped efficiently by allele specific PCR with the 9 primer pairs, in which 28 cultivars were identified for the first time and they were classified into 18 groups according to the genotype. The results also proved that the 9 S-allele specific primer pairs can efficiently identified S-genotype, which will be very useful for choosing pollinators or crossing parents in loquat production and breeding.

Keywords: specific pcr; loquat eriobotrya; eriobotrya japonica; allele specific; japonica lindl; primer pairs

Journal Title: Scientia Horticulturae
Year Published: 2017

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