LAUSR

&NA; The current study examine the effects of combined sprint and resistance training on serum total testosterone (TT), sex‐hormone‐binding globulin (SHBG) and cortisol (C), at rest, and in response to the Wingate Anaerobic‐Test (WAnT) in 21 and 41 years old men. Forty moderately‐trained men were randomly assigned to a young trained (YT), young control (YC), middle‐aged trained (MAT), and middle‐aged control (MAC) group. Before (P1), and after (P2) training, blood samples were collected at rest and after exercise. At P1, higher C and lower TT was observed in middle‐aged groups compared to younger ones (P < 0.05). At P2, basal TT increased significantly (P < 0.05) in MAT and the age‐difference was absent between trained groups (P > 0.05). Basal SHBG decreased significantly in YT at P2 (P < 0.05) but did not change in other groups from before to after training (P > 0.05). Free‐testosterone was significantly (P < 0.05) higher in young compared to middle‐aged groups at P1, but at P2, this age‐related difference disappeared between YT and MAT (P > 0.05). C post‐WAnT increased significantly for MAT only (P < 0.05) at P2, whilst no significant changes were observed in the other three groups (P < 0.05) at P2. In contrast, resting levels of C did not change in all groups at P2 (P > 0.05). The current study demonstrates that this training intervention may help increase steroids hormones in middle‐aged men and counteract the negative effect of age on TT and free testosterone.