LAUSR

Abstract P. major and P. lanceolata are two medicinal plants used extensively all over the world as remedies for a wide range of diseases. This study aimed to apply a sustainable recovery extraction to obtain bioactive extracts from the medicinal plants P. major and P. lanceolata . Different extraction methods were compared in terms of yield process, total phenolic content (TPC) and antioxidant and antibacterial activities. The methods used were: supercritical fluid extraction (SFE) with CO 2 pure and with ethanol as co-solvent, conducted at temperatures from 40 to 60 °C and pressures from 10 to 30 MPa, and by Soxhlet (SOX) and ultrasound-assisted extraction (UE) with different solvents. High extraction yields were obtained by SOX with methanol ( P. major ) and by UE with EtOH-H 2 O ( P. lanceolata ). The moderate-polar and polar solvents presented the best TPC values and antioxidant performance, included the supercritical extraction of P. major with 5.0% of ethanol as co-solvent (EC 50 276 ± 1). SC-CO 2 extract presented the best antibacterial activity, being more effective against the Gram-positive bacteria ( Bacillus cereus ). The results showed that the use of green recovery methods such as SFE and UE with polar solvents (EtOH-H 2 O and ethanol) provide high valuable extracts for Plantago species.