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Obtaining fatty acids from Mortierella isabellina using supercritical carbon dioxide and compressed liquefied petroleum gas

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Abstract The objective of this article was to provide the extraction yields, the kinetic parameters, and the fatty acids compositions in the oil obtained from freeze-dried cells of Mortierella isabellina… Click to show full abstract

Abstract The objective of this article was to provide the extraction yields, the kinetic parameters, and the fatty acids compositions in the oil obtained from freeze-dried cells of Mortierella isabellina . The oil was obtained by supercritical CO 2 (SFE-CO 2 ) and compressed liquefied petroleum gas (LPG) extractions in different conditions of temperature and pressure. According to the kinetic curves, the mass transfer rates, and the yields of extract, 80 °C/250 bar (SFE-CO 2 ) and 40 °C/20 bar (LPG) were the most favorable conditions. The highest yield was 4.45 wt.% (LPG). According to the fatty acids composition, SFE-CO 2 provided a slightly higher performance than compressed LPG. The highest concentration of fatty acids was 361 mg/g oil at 40 °C/150 bar. Stearic, cis -10-pentadecanoic, cis -10-heptadecanoic, oleic, linoleic and linolenic acids were the major fatty acids extracted. These results highlight the possibility of using pressurized fluids to break the cells for increasing the fatty acids extraction from a lipids-rich fungal biomass.

Keywords: compressed liquefied; mortierella isabellina; fatty acids; liquefied petroleum; petroleum gas

Journal Title: Journal of Supercritical Fluids
Year Published: 2017

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