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Principle of the BINACLE assay. (I.) BoNT molecules (grey) bind to immobilized receptors (blue and red) via their H-chains. Then the L-chains are released by reduction of the disulfide bonds.… Click to show full abstract
Principle of the BINACLE assay. (I.) BoNT molecules (grey) bind to immobilized receptors (blue and red) via their H-chains. Then the L-chains are released by reduction of the disulfide bonds. (II.) The supernatant with the liberated L-chains is transferred to a plate containing an immobilized substrate protein (brown). (III.) BoNT L-chains cleave the substrate protein, and the cleavage fragment is detected using a specific antibody.
Keywords:
binding cleavage;
cleavage;
binacle binding;
binacle;
assay vitro;
cleavage assay
Journal Title: Toxicon
Year Published: 2018
Link to full text (if available)
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Journal Title: Toxicon
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!