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Cell stimulation by focused electron beam of atmospheric SEM.

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Cell stimulation has been performed with a focused electron beam. To protect the live cells from the vacuum environment of the electron beam, the beam irradiated the ambient cells via… Click to show full abstract

Cell stimulation has been performed with a focused electron beam. To protect the live cells from the vacuum environment of the electron beam, the beam irradiated the ambient cells via a thin film. In this way, the cells were electrically stimulated with nanometre resolution in a non-contact process. The response of calcium ion concentration in a single HeLa cell after electron beam irradiation was examined. This technique has the potential to stimulate single ion channels, granules, and organelles.

Keywords: electron beam; cell stimulation; focused electron; beam

Journal Title: Ultramicroscopy
Year Published: 2019

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