LAUSR

The poor biodegradability of waste activated sludge (WAS) is widely regarded as one of the main bottlenecks in the fermentation of sludge and is attributed mainly to the complex nature of sludge. In this study, the physical structure and interfacial thermodynamics of sludge, which reflect its complex nature, were explored to reveal the effects of isoelectric-point (pI) pretreatment on enhancing the production of volatile fatty acids (VFA). It was observed that the maximum VFA production and the initial VFA production rate increased by 151.2% and 46.6%, respectively, after pI pretreatment, which indicates that pI pretreatment significantly improved the generation efficiency of VFA. The experimental results of 12-day acidogenic fermentation assays following pI pretreatment show that the maximum concentrations of soluble total organic carbon, soluble protein and soluble polysaccharide increased by 209.8%, 148.9% and 84.5%, respectively, and the maximal proportion of low molecular weight (<1 kDa) soluble organic substances increased by 92.4%, thus confirming that pI pretreatment can promote organic solubilisation and hydrolysis in sludge. The analyses of changes in the fractal dimension (Df), the spatial configuration of extracellular polymeric substances, and the interfacial non-covalent interaction energy of sludge during the fermentation process reveal that pI pretreatment can loosen the physical structure, promote the spatial extension of biopolymer molecular chains, and increase the driving forces of solid-liquid interfacial enzymatic reactions. It is thus hypothesised that these changes could be responsible for the high degree of organic solubilisation, hydrolysis and acidification of WAS, which is further confirmed by correlation analyses of the Df and interfacial free energy versus VFA production. These findings are expected to provide a possible means to improve the biodegradability of sludge via its pI to trigger dismantling of the sludge structure and increase the driving forces of interfacial enzymatic reactions.