LAUSR

BACKGROUND Today, Gamma knife radiosurgery plays an important role in neurosurgical procedures. Gamma knife radiosurgery has been used for many brain tumors and functional interventions. However, the Gamma knife treatment has a devastating impact on normal brain parenchyma surrounding the target point. It causes increased vascular permeability, vasodilatation, and swelling in endothelial cells. Ozone has antioxidant, antiapoptotic and anti-inflammatory effects in the body. As a result, we evaluated the radioprotective effects of ozone in rats undergoing Gamma knife radiation. MATERIAL AND METHOD In this study, a total of 24 Sprague-Dawley 250-300 g male rats were used. The experiment consists of 3 groups, each including eight rats. Rats were selected randomly. Control group did not receive any Gamma knife radiation. The other two groups received 50 Gy radiations. Group ozone was given ozone treatment after Gamma knife radiosurgery. Group nonozone did not receive any additional treatment. Twelve weeks after Gamma knife radiation, rats were sacrificed with high-dose anesthetic agents, and the tissues were prepared for evaluation. Slides were evaluated for necrosis, vacuolization, glial proliferation, and vascular proliferation with Hematoxyline-Eosin staining. Vascular endothelial growth factor and extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) were evaluated with immunohistochemical staining. RESULTS Statistically, vascular endothelial growth factor expression in glial tissue was found significantly less in group receiving ozone (χ2=15.00, df=4, p=0.005b control=ozone