LAUSR

Luteolin has been reported to impede migration via repressing tumor-associated macrophage-secreted chemokine ligand 2 expression. However, it's still unexplored whether luteolin represses the metastasis progression of leukemia cells via regulating the expression of C-X-C motif chemokine receptor 4 (CXCR4). Jurkat cells were stimulated by lactacystin and chloroquine after luteolin preincubation. C-X-C motif chemokine ligand 12 (CXCL12) was applied to induce migration of Jurkat cells after the cells were pre-incubated with luteolin (50, 100, 150, and 200 μM). CXCR4 was quantified by Western blot assay. Next, cell counting kit-8 (CCK-8) was used to detect cell viability. Apoptotic cells were observed with flow cytometry after staining. Migration assay was performed using a modified Boyden's chamber. We found that luteolin repressed CXCR4 expression at mRNA levels and restrained CXCL12-induced proliferation as well as migration. Furthermore, luteolin facilitated Jurkat cells apoptosis which was associated with the cleavage of caspase. Additionally, luteolin impeded the phosphorylated expression of PI3K, AKT, and ERK which was enhanced by CXCL12. In conclusion, luteolin exhibited anti-proliferation and anti-metastasis functions in CXCL12-treated Jurkat cells and inactivated PI3K/AKT and ERK signaling pathways via repressing the transcription of CXCR4.