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A two-step PCR with barcoded M13 Primers was used to enable pooling of 22 samples for a single SMRTbell library preparation, which was sequenced on the PacBio Sequel II and… Click to show full abstract
A two-step PCR with barcoded M13 Primers was used to enable pooling of 22 samples for a single SMRTbell library preparation, which was sequenced on the PacBio Sequel II and IIe Systems. HiFi reads (>QV20) were demultiplexed on SMRT Link v10.0 and clustered into haplotypes. The consensus reads of each haplotype were produced using the “pbaa” amplicon analysis from bioconda (https://github.com/PacificBiosciences/pbAA) and mapped to the human reference genome GRCh38 for the assignment of CYP2D6 types. Figure 1. Barcoded CYP2D6 Amplicons. LM, lower marker; UM, upper marker. The sample name on each electropherogram.
Journal Title: Molecular Genetics and Metabolism
Year Published: 2021
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