LAUSR

Abstract Diabetes mellitus (DM) is a metabolic disorder disease characterized by hyperglycemia. Long-term physiological hyperglycemia will aggravate the degree of non-enzymatic glycation (NEG) proteins. Because the abnormal changes of non-enzymatic process can cause a series of pathological variation, NEG proteins have attracted more and more attention. Mass spectrometry (MS) is an indispensable and powerful analytical tool for the qualitative and quantitative analysis of proteins due to its ultra-high sensitivity, excellent detection limits, and multi-component simultaneous analysis, and has been applied to the identification and qualification of NEG proteins. Herein, the principle of NEG proteins and the application of MS in the identification and quantitation of NEG plasma proteins and hemoglobin (Hb) in blood are reviewed. The forecast in the analysis of NEG proteins based on MS is also provided.