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Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis.

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Abstract It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to… Click to show full abstract

Abstract It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri (Mmc). Furthermore, little research on the characteristics of Mmc metabolism has been reported. In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression. Culture samples were harvested during logarithmic phase (PG3-1), stationary phase (PG3-2), decline phase (PG3-3) and late decline phase (PG3-4). Twelve RNA samples (three replicates for each of the four growth stages considered) from these cultures were collected and sequenced. Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes (P

Keywords: mycoplasma mycoides; subsp capri; phase pg3; mycoides subsp; pg3

Journal Title: Journal of Integrative Agriculture
Year Published: 2018

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