In recent years, biological specimen preparation using a cryo-focused ion beam (cryo-FIB) has become a key technique for investigating complex cellular structures in situ [1-3]. Cryo-FIB has enabled cryoelectron tomography… Click to show full abstract
In recent years, biological specimen preparation using a cryo-focused ion beam (cryo-FIB) has become a key technique for investigating complex cellular structures in situ [1-3]. Cryo-FIB has enabled cryoelectron tomography (cryo-ET) of plunge-frozen hydrated cells, revealing the cellular interior with sufficient resolution and contrast to study membrane-bound macromolecules in their native state [4-7].
               
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