LAUSR

Bridging light and electron microscopy through correlative light and electron microscopy (CLEM) enables imaging of protein localization within a detailed subcellular context [1]. Certain CLEM approaches, such as array tomography, nanofEM, are compatible with super-resolution imaging to localize the fluorophore with nanometer precision and access the subcellular context through scanning electron microscope (SEM) [2-4]. A recent study of the Drosophila central nervous system achieved single-synapse resolution by combining in resin fluorescent imaging and focused-ion beam SEM [5]. However, compared to TEM, high-resolution electron micrographs are difficult to obtain in SEM. Here, we present a CLEM approach that overcomes this obstacle by combining dSTORM [4] of resinembedded samples and TEM. As detailed below, following staining, super-resolution images were acquired on a slot grid and transmission electron micrographs were collected from the same grid. For proof of concept, we used an anti-actin antibody to detect sarcomere actin in Drosophila.