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Since its development in 1970s, fluorescence recovery after photobleaching (FRAP) became widely used method for measuring of protein mobility in live cells. The measurement starts by imaging of the initial… Click to show full abstract
Since its development in 1970s, fluorescence recovery after photobleaching (FRAP) became widely used method for measuring of protein mobility in live cells. The measurement starts by imaging of the initial distribution of the fluorescently-tagged protein of interest. Fluorescent molecules within the selected area, region of interest (ROI), are then irreversibly bleached by the exposure to a short pulse of high-intensity light. Subsequently, recovery of fluorescence within the bleached ROI is recorded. Evaluation of FRAP primarily involves the estimation of the parameters of appropriate mathematical model of the protein concentration profile evolution. Accessibility of FRAP increased with the commercialization of the confocal laser scanning microscope.
Journal Title: Microscopy and Microanalysis
Year Published: 2018
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