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Cryo-FIB Workflow for Fabricating Thin Lamellae Using TESCAN S8000G

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Understanding cellular ultrastructure and function, ideally in the native state requires a powerful analytical method. Cryo-electron tomography (cryo-ET) has become a well-established tool to study frozen vitrified specimens in a… Click to show full abstract

Understanding cellular ultrastructure and function, ideally in the native state requires a powerful analytical method. Cryo-electron tomography (cryo-ET) has become a well-established tool to study frozen vitrified specimens in a close-to-native state. A large proportion of samples in structural biology, such as plungefrozen eukaryotic cells, exceed the accessible dimensions (~500 nm) for TEM imaging and are highly topographic. Cryo-sectioning is the standard method used for thinning and slicing such samples. However, data quality is easily degraded due to artefacts caused by knife marks, compression or crevasses. [1]

Keywords: workflow fabricating; fib workflow; fabricating thin; cryo; cryo fib; thin lamellae

Journal Title: Microscopy and Microanalysis
Year Published: 2019

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