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Plant anatomy and development are difficult to study if researchers are restricted to the examination of 2D images, regardless of the resolution of the imaging technology being used. Generating detailed… Click to show full abstract
Plant anatomy and development are difficult to study if researchers are restricted to the examination of 2D images, regardless of the resolution of the imaging technology being used. Generating detailed 3D volumes of plant samples at cellular resolution would allow measurement and analysis of complicated plant cell biology in unprecedented detail. Electron microscopy (EM) allows high resolution imaging but there are practical limitations for 3D EM with regard to sample size and throughput [1]. There are excellent examples of researchers using conventional EM sample preparation techniques for improving contrast in X-ray microscopy (XRM) imaging of plant samples [2]. We have been adapting these protocols to take advantage of the relatively wide sample size range capabilities of the Zeiss Xradia 520 Versa to study plant cell biology with 3D XRM at near-EM level resolution.
Journal Title: Microscopy and Microanalysis
Year Published: 2019
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