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Completing the Picture: Compositional Data Obtained with Energy Dispersive X-ray Spectrometry Contributes Novel Information for Stained and Unstained Biological Samples

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Visualisation and analysis of biological samples has been a challenge throughout the history of electron microscopy with sample preparation and the absence or addition of contrasting agents playing a key… Click to show full abstract

Visualisation and analysis of biological samples has been a challenge throughout the history of electron microscopy with sample preparation and the absence or addition of contrasting agents playing a key role in the development of imaging methodology. Multi-colour electron microscopy (MCEM) combines ultrastructural electron data with elemental information about sample composition [1]. While energy dispersive x-ray spectrometry (EDX) has been used in materials science and biology for many decades, sample stability and detector sensitivity have limited the broader adoption in life sciences until recently [2].

Keywords: microscopy; ray spectrometry; dispersive ray; biological samples; energy dispersive; information

Journal Title: Microscopy and Microanalysis
Year Published: 2020

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