LAUSR

Single molecule localization microscopy (SMLM) was initially demonstrated nearly 15 years ago in a trio of seminal papers. Since then, it has been recognized an integral method among the broader superresolution imaging repertoire available to biologists. As such, a variety of 2D and 3D commercial implementations are currently available and in use. However, there continues to be obstacles to its wider dissemination and utilization – particularly in a open-access setting. Fundamentally, as with nearly any other optical microscopy technique, commercial SMLM typically suffers from poorer axial vs. lateral resolution. This can hamper some biological queries. More broadly, the technical requirements for sample preparation, image acquisition, as well as processing and data analysis can be substantial for many bioscience researchers.