LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Rapid Tilt-series Methods: The Future of Cryo-ET

Photo by iamthedave from unsplash

Cryo-electron tomography (Cryo-ET) has become an indispensable imaging technique to reveal the structures of biological complexes and the architectures of cells in a nearly native frozen-hydrated state. In Cryo-ET, a… Click to show full abstract

Cryo-electron tomography (Cryo-ET) has become an indispensable imaging technique to reveal the structures of biological complexes and the architectures of cells in a nearly native frozen-hydrated state. In Cryo-ET, a tilt-series is collected by tilting the sample around a tilt axis, acquiring 2D images at each discrete tilt angle. This tilt-series is then used to computationally reconstruct a 3D tomogram. One of the major challenges in Cryo-ET is throughput; a typical tilt-series takes an average of 30-40 minutes to collect, where the time is mostly spent on tracking tasks that ensure the target remains centered in the field of view (FoV).

Keywords: cryo; rapid tilt; tilt series; methods future; series methods

Journal Title: Microscopy and Microanalysis
Year Published: 2020

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.