LAUSR

Differential interference contrast (DIC) microscopy is a two-beam shearing interferometric technique of sensitive microscopy using videocameras. of the video-enhanced polarized light microscope video-enhanced PLM and DIC techniques greatly improved at the MBL. PLM and DIC are two complementary label-free techniques. While PLM generates an image of birefringence, the DIC technique produces a phase gradient image. For example, PLM is used for imaging spindles, and DIC is used for imaging chromosomes. these techniques have the same shortcoming. Their contrast is not quantitative, and it is strongly dependent on the specimen orientation. The has to mechanically rotate the specimen under investigation. The the quantitative orientation-independent differential polarized light microscope (LC Pol-Scope) This a quantitative of birefringence within of orientation-independent differential interference contrast (OI-DIC) on an