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The first small molecule fluorescent turn-on probes for detecting PDEδ protein were rationally designed, which showed reasonable fluo-rescent properties and can be applied into visualizing the PDEδ protein at the… Click to show full abstract
The first small molecule fluorescent turn-on probes for detecting PDEδ protein were rationally designed, which showed reasonable fluo-rescent properties and can be applied into visualizing the PDEδ protein at the living cell and tissue levels. The qPCR results showed that the mRNA expression of KRAS, PDEδ, AKT1, MAPK1, MEK7, RAF1 and mTOR were down-regulated by probes 1-3 through PI3K/AKT/mTOR and MAPK signal pathways. The probes also can down-regulate the protein level of pErk and tErk. Therefore, these small-molecule fluorescent probes are expected to be used into screening anti-pancreatic cancer drugs targeting on the PDEδ protein, as well as in better understanding the pathological and physiological roles of PDEδ protein.
Journal Title: Analytical chemistry
Year Published: 2020
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