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5-Carboxylcytosine (5caC) plays a vital role in the dynamics of DNA demethylation, sequencing of its sites will help us dig out more biological functions of 5caC. Herein, we present a… Click to show full abstract
5-Carboxylcytosine (5caC) plays a vital role in the dynamics of DNA demethylation, sequencing of its sites will help us dig out more biological functions of 5caC. Herein, we present a novel chemical method to efficiently label 5caC distinguished from other bases in DNA. Combined with bisulfite sequencing, 5caC sites can be located at single-base resolution. Further-more, dot blot assays have confirmed that 5caC-containing DNA isolated from HeLa cells was successfully labeled using our method. We are convinced that our strategy can be further applied to selectively tagging other carboxyl-modified bases and mapping their sites in RNA.
Journal Title: Analytical chemistry
Year Published: 2020
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