LAUSR

■ CONTENTS EV Biology 4740 History of Extracellular Vesicles and Classification 4740 Biogenesis of EVs 4742 Biogenesis of Microvesicles 4742 Biogenesis of Exosomes 4742 Molecular Compositions of EVs 4743 Heterogeneity of Exosomes 4743 Preanalytical Considerations of Sample Collection and Handling 4744 Cell Culture Conditioned Media 4744 Blood 4744 Pleural Effusions and Ascites 4745 Tear Fluid 4745 Saliva 4746 Urine 4746 Isolation and Enrichment of EVs 4746 Centrifugation-Based Methods 4746 Differential Ultracentrifugation 4746 Density Gradient Ultracentrifugation 4747 Polymer-Based Precipitation Methods 4747 Size-Based Separation Methods 4747 Size-Exclusion Chromatography (SEC) 4747 Ultrafiltration 4748 Tangential Flow Filtration 4748 Field Flow Fractionation 4749 Emerging Physical Isolation Technologies 4749 Viscoelastic Flow Sorting 4749 Deterministic Lateral Displacement (DLD) Sorting 4749 Acoustofluidic Technology 4749 Dielectrophoretic (DEP) Separation 4749 Thermophoresis Technology 4749 Affinity Binding-Based Isolation 4750 Immuno-Affinity 4750 Aptamer-Based Isolation 4751 Membrane Affinity Binding 4751 Conventional Methods for EV Characterization 4752 Morphological and Biophysical Characterization 4752 Microscopy Methods 4752 Dynamic Light Scattering and Nanoparticle Tracking Analysis 4752 Resistive Pulse Sensing 4752 Routine Biochemical Characterization 4753 Measurements of Total Molecular