ACCESS Metrics & More Article Recommendations ■ CONTENTS Functional Basics of Cas12 and Cas13 208 Nuclease Activity of Cas12a 208 Nuclease Activity of Cas13a 208 Quantitative Determination of trans-Cleavage Kinetics… Click to show full abstract
ACCESS Metrics & More Article Recommendations ■ CONTENTS Functional Basics of Cas12 and Cas13 208 Nuclease Activity of Cas12a 208 Nuclease Activity of Cas13a 208 Quantitative Determination of trans-Cleavage Kinetics of Cas Enzymes 209 trans-Cleavage Kinetics of Cas12 and Cas13 212 trans-Cleavage Kinetics of Cas12 and Its Contributing Factors 212 trans-Cleavage Kinetics of Cas13 and Its Contributing Factors 212 Nucleic Acid Detection Sensitivity Achieved by trans-Cleavage Activity of Cas Enzymes 213 Relations between the trans-Cleavage Kinetics and the Limit of Detection 213 Prediction of Performance Aided by Machine Learning 214 Challenges of trans-Cleavage and Signal Amplification in Live Cells 214 Concluding Remarks and Perspectives 214 Author Information 215 Corresponding Author 215 Authors 215 Notes 215 Biographies 215 Acknowledgments 215 References 216
               
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