LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

A DNA Tetrahedron Nanoprobe with Controlled Distance of Dyes for Multiple Detection in Living Cells and in Vivo.

Photo from wikipedia

Multicomponent quantitative detection in living samples is becoming increasingly important; however, the current detection strategy may cause fluorescence self-quenching and reduce the sensitivity of detection. To solve the problem, we… Click to show full abstract

Multicomponent quantitative detection in living samples is becoming increasingly important; however, the current detection strategy may cause fluorescence self-quenching and reduce the sensitivity of detection. To solve the problem, we develop a DNA tetrahedral nanoprobe to control the dyes distance for simultaneous detection of multiple analytes. Compared to mesoporous silica nanoparticles based nanoprobes, the DNA tetrahedral nanoprobes display enhanced fluorescence intensities due to partially avoiding the fluorescence resonance energy transfer. Confocal fluorescence images show that the nanoprobes are capable of detecting and visualizing pH and O2•- in living cells under a single wavelength excitation. In an inflammation model for mice, the nanoprobes simultaneously image the down-regulation of pH and up-regulation of O2•-. We expect that the current strategy can provide new opportunities in designing probes for multiplexed detection with reduced self-quenching and enhanced sensitivity.

Keywords: detection; detection living; fluorescence; living cells; dna tetrahedron; nanoprobe

Journal Title: Analytical chemistry
Year Published: 2017

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.