LAUSR

Dynamic tracking of the spatiotemporal coordination among various organelles to in-depth understanding of the mechanism of autophagy have attracted considerable attentions. However, the monitor of nucleoli participation in autophagy was somehow neglected. Herein, we report a RNA-targeted bio-probe (ADAP) with high selective permeability into nuclear pore complexes, which induced two-photon (TP) fluorescence "off-on" response by groove combination with RNA, dynamically monitoring the autophagy process among multi-organelles (nucleoli, mitochondria and mitochondria-containing lysosomes). This work provides a simple and convenient way to observe the dynamic behavior of multi-organelles during the autophagy process, which benefits for the understanding of cellular metabolism.