LAUSR

Because nitric oxide (NO) gas is an endogenously produced signaling molecule which is related to numerous physiological functions, numerous studies have been conducted to develop the NO delivery systems to take advantages for potential biomedical applications. However, NO is a reactive radical gas molecule which has very short life time and easy to transform to nitrogen oxide species by reaction with oxygen species. Therefore, it is necessary to develop the NO delivery carrier that releases NO gas to a topical area for the specific applications. In this study, laponite clay was introduced to fabricate the NO delivery carrier by the formation of a laponite-polyamine (LP-PAn) composites. The laponite clay and pentaethylenehexamine (PEHA) formed macromolecular structure by electrostatic interaction. And the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP-PAn composite. We investigated the conformation of the LP-PAn composite structure and the NO donor formation by zeta potential, x-ray diffraction UV-vis and fourier transform infrared (FT-IR) spectroscopy. Then, we analyzed the NO release profile. Additionally, we confirmed the applicability in biomedical applications via cell viability test and in vitro tube formation assay.