Bisulfite (HSO3-)/Sulfite (SO32-) is widely used as a food additive, but excessive use often leads to serious consequences, so the detection of HSO3-/SO32- is of great importance. In this paper,… Click to show full abstract
Bisulfite (HSO3-)/Sulfite (SO32-) is widely used as a food additive, but excessive use often leads to serious consequences, so the detection of HSO3-/SO32- is of great importance. In this paper, a novel 1,4-diethylpiperazine-modified coumarin-benzopyran derivative (probe QLP) has been synthesized and characterized. In PBS (10 mM, pH = 7.4), QLP displays good selectivity and is sensitive for HSO3-/SO32- over various analytes with fluorescent "OFF-ON" rapid responding (2 min), long-wavelength emission (600 nm), and a detection limit of 177 nM. With the treatment of HSO3-/SO32-, the color of the QLP solution obviously changes from blue-green to yellow, and the fluorescent color of QLP changes from colorless to amaranth. The fluorescence-enhanced mechanism is qualitatively evaluated by density functional theory calculations using the CAM-B3LYP/6-31G (d) method, which reveals that the photoinduced electron transfer leads to the fluorescence emission of the QLP-SO3H adduct. Importantly, nontoxic QLP can be used to detect HSO3-/SO32- in sugar, natural water samples, and living cells and localized to the mitochondria and monitor the mitochondrial HSO3-/SO32- level.