LAUSR

Three typical edible oils (palm oil, PO; leaf lard oil, LO; rapeseed oil, RO) and triacylglycerols (TAGs) (glycerol tripalmitate, GTP; glycerol tristearate, GTS; glycerol trioleate, GTO) were selected to conduct digestion experiments using fully designed in vitro digestion model. The evolutions in mean particle diameter, ζ-potential, and microstructural changes during different digestion stages were investigated. Free fatty acid (FFA) release extent and kinetics were monitored by pH-Stat method. The particle characterization of different lipids during passage through the GIT depended on lipid type and the microenvironment they encountered. Absorbed surface protein can hardly be the obstacle for pancreas lipase to catalyze lipid hydrolysis after gastric digestion. The maximum FFA release level and apparent rate constant in small intestine digestion stage of the three oils and TAGs were: PO > RO > LO, GTP > GTS > GTO, respectively. PO showed the highest FFA release level and rate mainly due to the short chain length saturated palmitic acid (C16:0) specifically located in the Sn-1, 3 positions of TAG molecules in palm oil, while the Sn-1, 3 positions of TAG molecules in RO and LO were mainly mono- or polyunsaturated fatty acids (C18:1 or C18:2), restricting the continuous hydrolysis reaction. These findings can provide some basic understanding of the digestion differences of different lipids, which may be useful for their nutritional and functional evaluation and the applicability in the food area.