LAUSR

The low phytic acid ( lpa) rice mutant Os-lpa-MH86-1, exhibiting a mutation-induced metabolite signature (i.e., increased levels of sugars, sugar alcohols, amino acids, phytosterols, and biogenic amines), was crossed with two commercial wild-type cultivars. The resulting progenies of generation F8 harvested at three independent field trials were subjected to a GC/MS-based metabolite profiling approach. Statistical assessments via multivariate and univariate analyses demonstrated that the environment had a strong impact on the metabolite profiles of the resulting progenies. In addition, the metabolites of homozygous lpa progenies were significantly influenced by the lipid profiles of the wild-type cultivars employed as the crossing parents. However, for each individual field trial, both the lpa trait and the mutation-specific metabolite signature were consistently expressed in the homozygous lpa mutant progenies of the two crosses. The data underline that cross-breeding can be employed as a tool to generate lpa progeny rice seeds stably exhibiting the mutation-induced metabolic traits.