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A blood glucose level lowering effect is postulated for polyphenols (PPs), which is in part attributed to the inhibition of α-amylase. To estimate structure-effect relationships chlorogenic acid (CA), phlorizin (PHL),… Click to show full abstract
A blood glucose level lowering effect is postulated for polyphenols (PPs), which is in part attributed to the inhibition of α-amylase. To estimate structure-effect relationships chlorogenic acid (CA), phlorizin (PHL), epigallocatechin gallate (EGCG), epicatechin (EC) and malvidin-3-glucoside (Mlv-3-glc) were used as inhibitors in an enzyme assay, based on the conversion of GalG2CNP by α-amylase. The detection of CNP was performed by UV/Vis spectroscopy. The data reveal that the inhibitor strength decreases as follows: EGCG > Mlv-3-glc > PHL ~ EC ~ CA. Detection of the substrate conversion by Isothermal Titration Calorimetry supports these results. All PPs showed mixed inhibition, except for CA and EGCG the competitive proportion was predominant. Investigations by Saturation Transfer Difference NMR revealed an interaction of PPs with α-amylase prevalently based on interactions with the aromatic or conjugated system. A correlation between the extent of the conjugated system and the IC50 of the PP could be found.
Journal Title: Journal of agricultural and food chemistry
Year Published: 2019
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