LAUSR

The distribution and deposition of fat tissue in different parts of the body are the key factors affecting the carcass quality and meat flavour of chickens. Adipose tissues from different locations display unique physiological, biochemical characteristics and gene expression patterns. To elucidate the transcriptomic changes of long noncoding RNAs (lncRNAs) in chicken intramuscular and abdominal adipocyte adipogenesis, we defined their transcriptome by Ribo-Zero RNA sequencing. A total of 11247 lncRNAs were observed in the adipocytes derived from IMF and AbF in chicken. Among them, we got 1624 differentiated expressed novel lncRNAs. A large amount of lncRNAs were enriched in various lipid metabolic processes and adipogenesis signaling pathways. Of these lncRNAs, lncAD is one of the most upregulated lncRNA and was co-expressed with several genes of PPAR signaling pathway. Here, we report that knockdown of lncAD inhibited its' upstream gene TXNRD1 expression in a cis-regulation manner, thus to decrease intramuscular preadipocytes adipogenic differentiation and promoted cell proliferation. This study revealed large transcriptomic differences between IMF- and AbF-derived preadipocyte differentiation. Additionally, the microenvironment of IMF- and AbF-derived preadipocyte may play significant roles in adipogenic differentiation. Collectively, our findings provide not only valuable evidence for the identification of lncRNAs in adipocytes differentiation, but also contribute to better understanding of the molecular mechanisms underlying tissues-specific fat deposition in poultry.