LAUSR

In this work, we have developed an all-in-one aptasensor based on enzyme driven three-dimensional DNA walker for the antibiotic detection. To overcome the drawback of time-consuming, high density substrate strands were anchored on the walking interface that accelerating the signal amplification efficiency. Such all-in-one design integrated the functionality of target recognition, signal amplification as well as signal output into a single probe. Upon adding kanamycin, the activated walking strand moved along the track by the stepwise cleavage of nicking enzyme, which resulting the enhancement of the fluorescence intensity of solution. Under the optimized conditions, the detection process was accomplished in 40 min with a low detection limit of 1.23 pM. This aptasensor was also applied in spiked milk samples with satisfactory recoveries of 97.76 to 105.33%, demonstrating an excellent stability and accuracy. Therefore, this all-in-one aptasensor shows great potential for the application in food safety.