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Four terpene synthases for biosynthesis of volatile terpenoids were identified from the transcriptome of Stellera chamaejasme L. flowers, including SchTPS1, SchTPS2, SchTPS3 and SchTPS4. Their functions were characterized by synthetic… Click to show full abstract
Four terpene synthases for biosynthesis of volatile terpenoids were identified from the transcriptome of Stellera chamaejasme L. flowers, including SchTPS1, SchTPS2, SchTPS3 and SchTPS4. Their functions were characterized by synthetic biology approaches in E. coli and in vitro enzymatic assays. SchTPS1, SchTPS2, and SchTPS3 are guaiene synthases, while SchTPS4 is an (E,E)-geranyl linalool synthase. Next, SchTPS1 and -guaiene 2-oxidase VvSTO2 were co-expressed in Saccharomyces cerevisiae to reconstruct the biosynthetic pathway of (-)-rotundone which is a unique aroma compound in fruits, vegetable and wines. This is the first report for construction of a (-)-rotundone-producing microbial platform.
Journal Title: Journal of agricultural and food chemistry
Year Published: 2020
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