LAUSR

In reversed-phase liquid chromatography (RPLC), retained analytes can diffuse faster along the hydrophobic surface of the stationary phase than when dissolved in the water (W)–acetonitrile (ACN) mobile phase. We investigate the surface diffusion of four typical aromatic hydrocarbon analytes in RPLC through molecular dynamics simulations in a slit-pore RPLC model consisting of a silica-supported, end-capped, C18 stationary phase and a 70/30 (v/v) W/ACN mobile phase. Our data show that the lateral (surface-parallel) diffusive mobility of the analytes goes through a maximum in the ACN ditch, an ACN-rich border layer around the terminal part of the bonded-phase chains, because the solvent composition there is more conducive to analyte mobility than the W-rich mobile phase. At their lateral mobility maximum, analytes have contacts with 12–15 bonded-phase groups, 5–6 ACN and 1–2 W molecules. The lateral mobility gain from surface diffusion decreases with analyte polarity first and size second (like and unlike r...