LAUSR

Altered cellular metabolism is one of the crucial hallmarks of glioma that deserves an exploration, as the metabolites act as direct indicators of protein function and genetic variations. The current study focused on metabolomic profiling of patients, from whom glioma specimens were obtained for identification of specific metabolites that could distinguish the low grade and high grade. In the current study 1 H NMR spectroscopy has carried out and the data were analysed by partial least squares discriminant analysis (PLS-DA) and orthogonal projection to latent structure with discriminant analysis (OPLS-DA). Pathway analysis was done to associate characteristic metabolites with the grades of sample using MetaboAnalyst 4.0 software based on KEGG metabolic pathways database. Distinctive metabolic profile among low and high grade gliomas with top fifteen characteristic metabolites that could discriminate these grades were identified on the basis of their VIP scores from the OPLS-DA model. The major altered metabolic pathways include: choline, taurine & hypotaurine, glutamate/glutamine, glutathione, and phenyl alanine/tyrosine which were found to be consistent with the particular grade of a sample. Our study clearly demonstrated a characteristic metabolic profile of an individual grades of glioma suggesting that an altered metabolism are consistent to the specific grades of glioma appreciation and which could lead to devise novel treatment strategies.