LAUSR

As a promising tool over the optical resolution limits, liquid electron microscopy is practically utilized to visualize the structural information of wet biological specimens, such as cells, proteins, and nucleic acids. However, functionality of biomolecules during their observations is still controversial. Here, we show feasibility of live cell electron microscopy using graphene veils. We demonstrate that the electron dose resistivity of living bacterial cells increases to 100-fold in graphene veils, and thus they maintain their structures and functions after electron microscopy experiments. Our results provide the guideline and show possibilities for electron microscopy imaging of live cells and functional biomolecules.