LAUSR

The capabilities of biosensors for a fast, economic and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity towards the adopted bioreceptors, a thin functional layer is required to enable coupling target biomolecules and to provide a good stability in the presence of sample matrix. In this paper, the generation of water-stable oxygen-rich plasma polymerized (pp) films deposited by atmospheric-pressure jet plasma for reliable immobilization of biomolecules is presented. Three types of pp films were developed and characterized. All of the obtained pp films were successfully used as matrix layer in the SPR immunosensors, which provided excellent level of sensitivity, stability and regenerability. The achieved results show that atmospheric pressure plasma polymerization is a powerful alternative method for the preparation of matrix layers for a wide range of applications in the biological field.