LAUSR

The encapsulation of multiple enzyme/nanoenzyme systems within mental-organic frameworks (MOFs) shows great promise for a myriad of practical applications. Herein, two sequential biocatalysts, oxidase and Hemin, were coupled together with close proximity by a bifunctional polymer, poly(1-vinylimidazole) (PVI), and encapsulated into MOFs. As a demonstration of the power of such protocol, glucose oxidase&PVI-Hemin encapsulated in ZIF-8 showed significant enhancement of bioactivity for cascade reaction compared to its counterpart without PVI. For colorimetric assay of glucose, it showed a low limit of detection of 0.4 μM (S/N=3), high selectivity, and excellent stability. Since there are numerous biocatalysts that can readily be coupled and encapsulated into MOFs, a myriad of interesting properties can be simply realized by encapsulating different sequential biocatalysts.