LAUSR

Protein imprinting technology is of interest in drug delivery, biosensor, solid-phase extractions and so forth. However, the efficient recognition and separation of proteins have remained challenging to date. Toward that, under the assistance of Ni2+-BSA directional coordination strategy, magnetic bovine serum albumin (BSA) imprinted materials had been synthesized via dopamine self-polymerization on hollow Fe3O4@mSiO2 microspheres (mSiO2 referred to mesoporous silica). The well-defined imprinted microspheres possessed more satisfactory adsorption capacity (266.99 mg/g), enhanced imprinting factor (IF, 5.45) and faster adsorption saturation kinetics (40 min) for BSA, superior to many previous reports. Benefiting from the coordinate interaction between Ni2+ and BSA, these fabricated microspheres exhibited excellent specificity not only in individual and competitive protein rebinding samples, but also in bovine serum. Combining with directional coordination method, the magnetic imprinted composite materials to selectively capture target proteins could provide promising potential in applications.