LAUSR

A simple process is developed for one step preparation of dual-compartmental alginate microcapsules with controlled size and structure from microfluidic-generated water-in-water-in-oil (W/W/O) emulsion droplet. Unlike other methods that rely on transient W/W/O emulsion droplet, we introduce the aqueous two-phase system (ATPS) to form stable W/W/O emulsion droplet as template for preparing dual-compartmental alginate microcapsules. Two different bioactive molecules are able to be spatially confined encapsulated in the shell and core of alginate microcapsules due to partitioning effect of ATPS and high viscosity of alginate solution. Moreover, an enzyme cascade reaction with spatial confined glucose oxidase (GOX) and horseradish peroxidase (HRP) in the shell and core of alginate microcapsules confirms its excellent biocompatibility and high activity. This method provides a green platform for enzyme catalyzed tandem reactions and controlled sequential release of multiple drugs based on alginate microcapsules.