LAUSR

We designed a novel luminescent metal-organic framework (LMOF) named CTM through a simple one-pot solvothermal method. CTM was synthesized by using the emerging electrochemiluminescent (ECL) material (4,4',4'',4'''-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) as organic ligand and Ce(Ⅲ) as metal node. We found that CTM not only has the remarkable ability to emit light but also has a uniform "sandwich biscuit" shape and suitable nanoscale size, which are promising for further applications. We also applied CTM to construct a novel ECL immunosensor and achieve the sensitive detection of the proprotein convertase subtilisin/kexin type 9 (PCSK9), a biomarker related to cardiovascular diseases (CVDs). To further amplify the ECL signal of CTM, a novel dual-amplified signal strategy was established by inducing polyamidoamine dendrimer (PAMAM) and gold nanoparticle (AuNPs). Importantly, we firstly proved that the ECL signal of CTM/S2O82- system could be enhanced by PAMAM electric field. Since the electron transfer rate was accelerated by AuNPs layer, this ECL signal was further enhanced in AuNPs-modified electrodes. The ECL immunosensor showed desirable performance for PCSK9 analysis within a detection range of 50 fg mL-1 to 10 ng mL-1 and a low LOD of 19.12 ± 2.69 fg mL-1. Real sample detection suggested that the immunosensor holds great potential for clinical serum samples analyzing.